Nextera DNA文库制备试剂盒提供最快捷简单的工作流程,能够在不到90分钟的时间内制备测序即用型文库,所需的手动操作时间不到15分钟。使用Nextera试剂盒制备的样本与所有Illumina测序仪都兼容。
借助Nextera技术,使用标准实验室设备即可在一个步骤中将DNA同时片段化并加上测序接头进行标记。此操作流程适用于数量有限的珍贵样本,只需要50纳克的DNA输入。
Assay Time | 90 minutes |
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Hands-On Time | 15 minutes |
Input Quantity | 50 ng genomic DNA (compatible with most large DNA genomes) |
Mechanism of Action | Enzymatic Fragmentation |
Multiplexing | Up to 96 available indexes |
Species | Human, Mammalian, Mouse, Other, Plant, Rat |
Variant Class | Insertions-Deletions (indels), Single Nucleotide Polymorphisms (SNPs), Structural Variants |
Method | Whole-Genome Sequencing |
System Compatibility | HiSeq 2500, HiSeq 3000, HiSeq 4000, MiniSeq, MiSeq, NextSeq 500, NextSeq 550 |
Specialized Sample Types | Low Input, Single Cells |
Technology | Sequencing |
Automation Capability | Liquid Handling Robots |